Flow core facilities play a pivotal role in enabling scientific breakthroughs, providing centralized expertise and technologies to all researchers within their institute. Bio-Rad is committed to helping flow core facilities serve their users’ existing and emerging needs with the instruments and reagents to advance their research. The high‑parameter, high‑throughput, automation‑capable ZE5 Cell Analyzer delivers high quality data from limited samples in a fraction of previous acquisition times, with hands-free capabilities to free up core lab staff’s on-instrument time.
The compact S3e Cell Sorter brings cell sorting to individual users and lets them isolate pure, viable cells anytime for CRISPR gene editing, next‑generation sequencing, and single-cell analysis.
Pair these instruments with our ever‑expanding range of thousands of high quality antibodies and reagents, including the innovative new range of StarBright Dyes for high multicolor resolution, to get reproducible, trustworthy results.
A Flow Cytometer Built for Automation
High Speed, High Throughput, High Parameter
The ZE5 Cell Analyzer is the only flow cytometer with the ideal combination of production throughput, smart sample-handling, and superior speed and sensitivity. Analyze up to 100,000 events per second with no data abort, expand the depth of your data to up to 27 colors, and use the integrated universal sample loader to freely switch between tube racks and plates. With its device-agnostic API, the ZE5 can be integrated into a robotic work cell for automated analysis; and with its external fluidics upgrades, the ZE5 enables unattended overnight operation for maximal productivity.
Quantification of T‑Cell Subsets
Learn how T‑cells were profiled after a stem cell transplant, using a 21‑color panel.
Get More from Your Experiments
See how the ZE5 lets you study rare cells and small samples with confidence.
Can we answer questions about the ZE5?
Webinars and Customer Testimonials
Flow Cytometry for Exosome Research
Read how flow cytometry can answer more exosome questions than other techniques.
Improve the Quality of Your Cell Analysis
Discover how the ZE5 Cell Analyzer can significantly expand your cell analysis capabilities compared to traditional flow cytometers.
Benefits of Flow Cytometry in Immuno-Oncology
See how flow cytometry benefits the development of more successful cancer therapies.
Small Particle Detection and Analysis on the ZE5 Cell Analyzer
Review the data showing direct exosome detection by flow cytometry.
Multiparameter Panel for Immunophenotyping Leukocytes
Explore the 19-color white blood cell panel developed using the ZE5 Cell Analyzer's 30 analysis parameters for...
Flow Experiments Come Alive
See a variety of experimental applications that feature the ZE5 Cell Analyzer and Bio-Rad antibodies and reagents.
The Ins and Outs of the ZE5 Cell Analyzer
Read about how the ZE5 Cell Analyzer was conceptualized and the many benefits it offers.
Analysis of Mixed Microbial Populations and Immunotyping of Peripheral Blood
See how the ZE5 Cell Analyzer helps perform accurate high-dimensional multicolor analysis and large panel...
ZE5 Cell Analyzer Publications List
Discover the fast-growing list of ZE5 publications
Automation of HT Flow with the ZE5
See what should be considered and what can be accomplished when automating your flow cytometer.
No Experience? No Problem. The cell sorter anyone can use.
Core lab users who need lots of fresh, viable cells for next-gen sequencing and single-cell analysis need a cell sorter that is so easy-to-use your researchers can use it themselves. This frees you up to focus on the more complex cell sorting projects, and gives them the ability to isolate viable cell populations when they need it. The easy-to-use yet powerful S3e Cell Sorter was designed for novice users, but engineered to enable high speed sorts with maximum recovery and viability.
Designed for Rare Cells and Precious Samples
Sorting rare cells and precious samples requires a powerful yet fast cell sorter. Without the S3e Cell Sorter in your lab, your samples are at risk of cell death. Find out how fast and easy it is to preserve your rare and precious samples when sorting with an S3e Cell Sorter, right in your lab.
Cell Sorting Testimonials
Have a question or want to learn more?
Enhance the Power of Droplet Digital Technology with the S3e Cell Sorter
Bio-Rad’s Droplet Digital technology enables precise, sensitive analysis of nucleic acids. Combining the S3e with ddPCR or ddSeq lets users quantify DNA/RNA as well as uncover heterogeneity in targeted cell subpopulations.
S3e and Single‑Cell Analysis
Finding heterogeneity within rare cell populations by pairing single‑cell RNA‑Seq with cell sorting.
Pure, Viable Stem Cell Populations
Isolating cancer stem cells from tiny biopsies, stem cells from blood, and iPSCs or ESCs from culture — with high purity and viability — is a challenge that users can meet with the S3e Cell Sorter.
Discuss Your Workflow Needs with a Specialist.
With its unique feature set and technical ease of use, the automated S3e Cell Sorter allows you to perform 1–4 color sorts in less than 30 minutes and greatly enhances your experimental success. The S3e Cell Sorter utilizes stream-in-air fluid dynamics for a gentler high-speed sort while automated setup and email or text message notifications let the user truly multitask during a sort.
ProDrop Technology Poster
Learn about a novel approach to automated drop delay measurement and monitoring.
Fluorophore and Dye Compatibility Charts
The S3e Cell Sorter accommodates the majority of fluorophores and dyes commonly used in flow cytometry today.
See How the S3e Cell Sorter Is Being Used to Drive Discovery
Browse our publication list to see how the S3e enables advances in cancer, stem cell, and immunology research.
Reliable Single-Cell Sorting
Accurate isolation of individual cells is essential for single-cell analysis techniques. Learn how precisely the S3e Cell Sorter can sort single cells.
Isolate Stably Transfected Cells
The S3e lets you isolate an incredibly pure subpopulation of cells right in your own lab. Download this application note to see just how pure.
Need more information?
Experiments Come Alive with Flow AntiBodies and Reagents
Proper sample preparation along with specific antibodies and reagents are key to having the confidence to experiment and expand your flow cytometry panels. We have an ever expanding range of antibodies and reagents to support your research and get you started right away. Below you can access invaluable flow cytometry guides, webinars, protocols, and tips to aid in assay design and data analysis.
Introducing StarBright Dyes, from Bio-Rad, high performance fluorophores specially developed for flow cytometry.
Steps to Success Poster
Key steps to consider while planning a flow cytometry experiment.
ZE5 Reagent Reference Guide
View at a glance how reagents map to the ZE5 Cell Analyzer’s lasers and filters.
Multicolor Panel Building
Use our reference chart to find compatible fluorophores for your experiments.
Build multicolor flow cytometry panels
Flow Cytometry Webinars
Antigen Density for Human and Murine Surface Markers
This guide lists the antigen density of some common human and murine markers to help you choose the right...
Antibody Titration in Flow Cytometry
Guide on why titration is an essential step to optimize your staining and how to do it.
Gain Confidence to Design Flow Cytometry Experiments
A Novice’s step-by-step guide to starting flow cytometry, with useful information and tips to help you get the best results.
Successful Setup Tips
Tips for maximizing cell separation and isolation prior to downstream processing.
Antibodies Conjugated to Fluorophores Reference
Reference guide of available fluorophores
Intracellular Flow Cytometry Tips
Tips for fixation and permeabilization prior to staining of intracellular proteins.
Cell Frequency Tables
Determine optimal starting cell numbers for staining and cell sorting using our cell frequency tables.
Multicolor Flow Panel Tips
Tips to keep in mind when designing multicolor flow panels.
No Compensation Panels
Examples of panels featuring common surface markers for human peripheral blood
Flow Cytometry Shorts: Webinar Series: Controls
Presented by: Sharon Sanderson, PhD, Flow Cytometry Research Associate at Bio‑Rad
Gates, Plots, and Regions
A guide to analyzing common flow cytometry plots.
A handy checklist that describes essential controls for a successful experiment.
Find out how isotype controls allow you to control for background staining.
ReadiDrop Cell Viability Assays
Improve the accuracy of your results by excluding dead cells using these reagents.
VivaFix Cell Viability Assays
Sensitive cell viability assays compatible with cell fixation.
CytoTrack Cell Proliferation Assays
Stain live cells and track up to ten generations.
PureBlu Nuclear Staining Dyes
Single-step dilution format for staining nuclei for cell imaging.
10 Things to Do Before Sorting
Tips for improving cell recovery when using a cell sorter.
Single-Cell Sorting with the S3e Cell Sorter
A simple workflow to verify the reliability of the S3e in sorting single cells.
Sorting Mesenchymal Stem Cells with the S3e Cell Sorter
Isolate rare MSCs from a mixed bone marrow culture with excellent sort efficiency and postsort purity.
Single-Cell Gene Expression Profiling by Multiplex RT-qPCR
A validated protocol that enables fast and accurate analysis of isolated single cells.