The quality of flow cytometry data is often dependent on the quality of your sample. The viability of your sample is important as dead cells will have high autofluorescence and bind antibodies nonspecifically.
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Sharon Sanderson, PhD, is a Flow Cytometry Research Associate for Bio-Rad. She obtained her degree in Molecular Biology from The University of Manchester before moving to The Institute of Cancer Research obtaining a PhD in Cell Biology from the University of London, with her thesis focusing on Angiogenesis. Sharon then moved to the University of Oxford where she worked for 13 years, initially continuing with research in the cancer field before moving into immunology research.