Every droplet tells a story. Each webinar shows you how.
Bio-Rad is pleased to invite you to participate in an ongoing webinar series exploring the extraordinary possibilities of Droplet Digital PCR. Each webinar in the series is hosted by a world-class scientist from the Digital Biology
Center at Bio-Rad. By registering for the series you will have access to all archived content and upcoming events.
Fill out the form below to receive complete access to previously recorded sessions and to be notified of the exciting topics we’ll be covering when the series resumes.
Learn about Droplet Digital PCR
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Archived Webinars (available for on-demand viewing) |
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Ultra-sensitive quantification of genome editing events using Droplet Digital™ PCR (ddPCR™) Jennifer R. Berman, PhD, Staff Scientist at the Digital Biology While powerful, the efficiency of gene editing is cell type–dependent and often low (<5%), particularly in primary cells or iPSCs. Here we demonstrate the use of ddPCR technology for ultra-sensitive detection of edited alleles. Part 2 of 2: Francisco Bizouarn, Global Digital Applications Specialist at the Digital Biology Center Technical overview of Droplet Digital PCR (ddPCR) technology and workflow and technical discussion of key ddPCR applications, including copy number variation, rare mutation detection, and absolute quantification of nucleic acids. Part 1 of 2: Counting on Droplet Digital™ PCR — George Karlin-Neumann, PhD Director of Scientific Affairs at the Digital Biology Center Discussion of a wide range of Droplet Digital PCR applications, including monitoring of cancer therapies, identification of under-recognized somatic mosaicisms, and high-level multiplexing for rare mutation detection and single-cell gene expression analysis. Droplet Digital PCR (ddPCR™) Applications for Molecular Pathology and Liquid Biopsy Dawne Shelton, PhD, Senior Scientist at the Digital Biology Center Tracking of cell-free tumor DNA during disease progression and treatment monitoring; early detection of tumor cfDNA in blood; detection of copy number amplifications; detection of KRAS mutations using multiplex screening Applying Droplet Digital™ PCR to the Study of Environmental Samples Yann Jouvenot, PhD, Staff Scientist at the Digital Biology Center Detection of viruses and microbes in environmental samples; monitoring of invasive species; investigation of malaria mosquito genetics Digital PCR Tips and Tricks: Assay Design Dianna Maar, PhD, Senior Scientist at the Digital Biology Center Assay design guidelines; identification and resolution of assay design problems; assays design for detection of difficult targets; ddPCR using FFPE, cell-free DNA, and other fragmented or low-yield sample types The Power of Partitioning: Droplet Digital™ PCR George Karlin-Neumann, PhD Director of Scientific Affairs at the Digital Biology Center Introduction to the concept, workflow, and performance of Droplet Digital PCR; summary of diverse published applications Droplet Digital™ PCR for Cancer and Liquid Biopsy Studies Dawne Shelton, PhD, Senior Scientist at the Digital Biology Center Techniques and methods that enable identification of cancer subtypes, optimization of drug treatment plans, monitoring of residual disease, and study of tumor evolution Gene Copy Number Variation Analysis Jennifer R. Berman, PhD, Staff Scientist at the Digital Biology Accurate and reproducible copy number determination using ddPCR, a simple, cost-effective workflow amenable to high throughput Droplet Digital™ PCR for Next Generation Sequencing Library Validation Nicholas J. Heredia, PhD, Staff Scientist at the Digital Biology Center Cross-validation of NGS data with respect to rare species targets; quantification of NGS libraries to optimize sequencing performance Enabling High-Resolution Gene Expression and miRNA Analysis with ddPCR™ Yann Jouvenot, PhD, Staff Scientist at the Digital Biology Center Gene and miRNA expression analysis; recommendations for assay design; ddPCR compatibility with various RT kits; single-well multiplexing Droplet Digital™ Applications: Phasing of Genetic Variants for CFTR Haplotyping Jack Regan, PhD, Group Leader, Droplet Digital PCR Applications at the Digital Biology Center Discussion of how Droplet Digital PCR can be used to determine whether genomic sequences are physically linked, enabling us to assess disease carrier status, understand differences in allele-specific expression, and clarify genome architecture. |