Every droplet tells a story.
Every webinar shows you how.

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The Droplet Digital™ PCR (ddPCR™) webinar series

The Droplet Digital PCR Webinar Series is an ongoing webinar series hosted by world-class scientists from the Digital Biology Center at Bio-Rad. Webinar topics range from an introduction to ddPCR technology to in-depth discussions of such ddPCR applications as the tracking of cell-free tumor DNA, characterization of copy number variation, and monitoring of the spread of invasive species. Whether you’re new to ddPCR or looking to get the most out of your system, we invite you to listen, learn, and then ask how Droplet Digital PCR can help rewrite your research story.
Learn about Droplet Digital PCR

Archived Webinars (available for on-demand viewing)

Jennifer Berman

Ultra-sensitive quantification of genome editing events using Droplet Digital™ PCR (ddPCR™)

Jennifer R. Berman, PhD, Staff Scientist at the Digital Biology

While powerful, the efficiency of gene editing is cell type–dependent and often low (<5%), particularly in primary cells or iPSCs. Here we demonstrate the use of ddPCR technology for ultra-sensitive detection of edited alleles.

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Francisco Bizouarn

Part 2 of 2: Precise Counting of Targeted Nucleic Acids Has Never Been Easier

Francisco Bizouarn, Global Digital Applications Specialist at the Digital Biology Center

Technical overview of Droplet Digital PCR (ddPCR) technology and workflow and technical discussion of key ddPCR applications, including copy number variation, rare mutation detection, and absolute quantification of nucleic acids.

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George Karlin-Neumann

Part 1 of 2: Counting on Droplet Digital™ PCR — More Applications than Anyone Ever Dreamed Of

George Karlin-Neumann, PhD Director of Scientific Affairs at the Digital Biology Center

Discussion of a wide range of Droplet Digital PCR applications, including monitoring of cancer therapies, identification of under-recognized somatic mosaicisms, and high-level multiplexing for rare mutation detection and single-cell gene expression analysis.

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Dawne Shelton

Droplet Digital PCR (ddPCR™) Applications for Molecular Pathology and Liquid Biopsy

Dawne Shelton, PhD, Senior Scientist at the Digital Biology Center

Tracking of cell-free tumor DNA during disease progression and treatment monitoring; early detection of tumor cfDNA in blood; detection of copy number amplifications; detection of KRAS mutations using multiplex screening

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Yann Jouvenot

Applying Droplet Digital™ PCR to the Study of Environmental Samples

Yann Jouvenot, PhD, Staff Scientist at the Digital Biology Center

Detection of viruses and microbes in environmental samples; monitoring of invasive species; investigation of malaria mosquito genetics

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Dianna Maar

Digital PCR Tips and Tricks: Assay Design

Dianna Maar, PhD, Senior Scientist at the Digital Biology Center

Assay design guidelines; identification and resolution of assay design problems; assays design for detection of difficult targets; ddPCR using FFPE, cell-free DNA, and other fragmented or low-yield sample types

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George Karlin-Neumann

The Power of Partitioning: Droplet Digital™ PCR

George Karlin-Neumann, PhD Director of Scientific Affairs at the Digital Biology Center

Introduction to the concept, workflow, and performance of Droplet Digital PCR; summary of diverse published applications

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Dawne Shelton

Droplet Digital™ PCR for Cancer and Liquid Biopsy Studies

Dawne Shelton, PhD, Senior Scientist at the Digital Biology Center

Techniques and methods that enable identification of cancer subtypes, optimization of drug treatment plans, monitoring of residual disease, and study of tumor evolution

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Jennifer Berman

Gene Copy Number Variation Analysis

Jennifer R. Berman, PhD, Staff Scientist at the Digital Biology

Accurate and reproducible copy number determination using ddPCR, a simple, cost-effective workflow amenable to high throughput

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Nicholas Heredia

Droplet Digital™ PCR for Next Generation Sequencing Library Validation

Nicholas J. Heredia, PhD, Staff Scientist at the Digital Biology Center

Cross-validation of NGS data with respect to rare species targets; quantification of NGS libraries to optimize sequencing performance

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Yann Jouvenot

Enabling High-Resolution Gene Expression and miRNA Analysis with ddPCR™

Yann Jouvenot, PhD, Staff Scientist at the Digital Biology Center

Gene and miRNA expression analysis; recommendations for assay design; ddPCR compatibility with various RT kits; single-well multiplexing

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John Regan

Droplet Digital™ Applications: Phasing of Genetic Variants for CFTR Haplotyping

Jack Regan, PhD, Group Leader, Droplet Digital PCR Applications at the Digital Biology Center

Discussion of how Droplet Digital PCR can be used to determine whether genomic sequences are physically linked, enabling us to assess disease carrier status, understand differences in allele-specific expression, and clarify genome architecture.

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