Bio-Rad offers a broad range of products to meet all your qPCR needs. Our patented* Sso7d fusion enzyme technology enables our supermixes to perform consistently under a variety of conditions — even in the presence of many common PCR inhibitors. When combined with our universal reference dye, the result is a qPCR supermix that performs under a wide variety of conditions on any real-time instrument.
Choose the right reagent for your real-time PCR needs:
Superior qPCR Performance
SsoAdvanced™ Universal SYBR® Green Supermix
> Learn more
qPCR Directly From RNA
iTaq™ Universal SYBR® Green One-Step Kit
Skip RNA Purification
SingleShot™ Cell Lysis RT-qPCR Kits
Our Sso7d fusion polymerase consists of a proprietary polymerase coupled to a double-stranded DNA binding protein that stabilizes the polymerase-template complex for increased processivity. By keeping the polymerase bound to the template, these supermixes retain their high sensitivity and efficiency even in the presence of PCR inhibitors or during amplification of challenging templates.
Our SsoAdvanced and iTaq lines of universal supermixes contain a proprietary reference dye formulation that allows them to produce properly normalized fluorescence data on any real-time PCR instrument (ROX-dependent and -independent).
Explore our many qPCR resources
Related Products> qPCR family of products
> Gene expression assays
> Reverse transcription reagents
Articles> Too Many Questions, Too Little Sample: Developing a Real-Time PCR Workflow
for Monitoring Gene Expression in
Interactive Tutorials> Understanding Real-Time PCR
> Understanding Real-Time PCR Supermixes
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.