Understand the factors that are crucial to successful protein purification

Join Bio-Rad for an educational series that explores the many factors that go into designing and optimizing conditions for performing successful purifications. We will discuss strategies and best practices using chromatography resins that can be
used for both small- and large-scale purifications. Register for the next webinar in our series or view a previously recorded
webinar on demand.

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DOE on Nuvia cPrime Resin — Simplifying Method Optimization on Mixed‑Mode Resins

Chelsea Pratt, PhD, Applications & Collaborations Manager at Bio-Rad Laboratories

Presentation 1: October 4, 9:00 AM PDT (San Francisco) | October 4, 5:00 PM BST (London)
Presentation 2: October 4, 7:00 PM PDT (San Francisco) | October 5, 10:00 AM SGT (Singapore)

With the increasing number of monoclonal antibody therapeutics entering clinical trials, there is an increased need for products that facilitate contaminant and aggregate removal. Multiple resins are typically screened to determine the optimal purification strategy. Of late, there has been an uptick in the usage of mixed-mode resins in purification strategies due to their multimodal interactions with target proteins. Since mixed-mode resins interact with proteins in multiple ways, it is difficult to predict the binding and elution conditions based on the pI of the protein. A simple DoE setup can be used to determine the optimal purification conditions with mixed-mode resins. This presentation from Bio-Rad will show you how to decipher these conditions, using basic lab equipment, with minimal sample, resin, and time. Mixed-mode resins can thus be easily used for process development of difficult to purify proteins.

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Virus Purification Strategies and Considerations

Chelsea Pratt, PhD, Applications & Collaborations Manager at Bio-Rad Laboratories

On the Quest to Purify a Large Complex Virus

The number of gene therapy–based treatments has grown significantly since they first appeared several decades ago. There is an optimism about the potential to develop cures for diseases such as cancer and AIDS. The ability to use viruses as vehicles for delivering genes to target cells is one of the biggest contributors to gene therapy. Adenovirus (Ad) and adeno-associated virus (AAV) have become the vehicles of choice, and AD and AAV studies account for over 25% of all ongoing gene therapy trials. The challenge, however, is producing sufficient quantities for therapeutic use of pure clinical-grade virus, which is required to ensure biosafety. One of the main barriers to achieving high purity levels is the size and complexity of adenovirus, which makes traditional virus purification methods inefficient. In addition, the virus has thousands of charge variants, making it difficult to establish well-defined binding and elution conditions. Therefore purification protocols and strategies used for small- and mid-sized viruses fall short when it comes to adenoviruses. Alternative strategies are needed for achieving efficient adenoviral purification. This webinar will provide a snapshot of the resins considered, and the reasons for their selection, in order to achieve the required yield and purity.

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Increase Yield and Reproducibility While Saving Time by Automating Recombinant Protein Purification

Philip Chapman, MSc, Senior Global Product Manager

Session 1: July 31, 2018, 8:00 AM Pacific time
Session 2: July 31, 2018, 6:00 PM Pacific time

Forty percent of recombinant proteins that are purified use a histidine tag for easy capture. This webinar covers how to automate purification of histidine-tagged proteins to increase reproducibility, recovery, and efficiency. We will share how purification conditions can be optimized before automating tandem or multidimensional purification and how manual steps that cause loss of precious proteins and take more time, like dialysis, collection, and reinjecting samples, can be removed.

We will present data showing a simplified way to scout for ideal purification conditions and the development and validation of a completely automated four-step purification scheme. The final purification scheme reduces a 3–4 day process to 11.5 hours from start to finish, all while improving yield and comparable purity.

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Large Biomolecule Purification with a New High-Performance Anion Exchange Resin

Hana Kim, PhD, Global Product Manager, Process Chromatography at Bio-Rad Laboratories
Jiali Liao, PhD, Principal Scientist, Process Chromatography at Bio-Rad Laboratories

Session 1: October 2, 2018, 9:00 AM Pacific time, 6:00 PM Central European time
Session 2: October 5, 2018, 1:00 PM Singapore time

Large molecule purification can be difficult due to their size. This leads to slow diffusion and mass transfer kinetics of the molecules into the pores of traditional chromatography resins, which decreases their binding capacity and resolution. In this webinar, Drs. Kim and Liao will introduce the design features of the Nuvia HP-Q bead matrix that help overcome these purification challenges.

Case studies on plasma protein and virus purification will be presented, providing examples of plasma protein and virus purification. These applications demonstrate the increased productivity and overall improved process economics for large molecule purification.

Overview:

Challenges in large molecule purification
Introduction to Nuvia HP-Q
How Nuvia HP-Q works and its benefits
Case studies

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A New Mixed-Mode Media for Biomolecule Purification

Daniel Yoshikawa, PhD, Global Product Manager, Process Chromatography
Mark A. Snyder, PhD, R&D Applications Manager, Process Chromatography

Session 1: October 16, 2018, 9:00 AM Pacific time, 6:00 PM Central European time
Session 2: October 17, 2018, 1:00 PM Singapore time

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Protein Purification webinar series