Jennifer R. Berman, PhD, Staff Scientist at the Digital Biology Center While powerful, the efficiency of gene editing is cell type–dependent and often low (<5%), particularly in primary cells or iPSCs. Here we demonstrate the use of ddPCR technology for ultra-sensitive detection of edited alleles.
Francisco Bizouarn, Global Digital Applications Specialist
at the Digital Biology Center Technical overview of Droplet Digital PCR (ddPCR) technology and workflow and technical discussion of key ddPCR applications, including copy number variation, rare mutation detection, and absolute quantification of nucleic acids.
George Karlin-Neumann, PhD
Director of Scientific Affairs at the Digital Biology Center Discussion of a wide range of Droplet Digital PCR applications, including monitoring of cancer therapies, identification of under-recognized somatic mosaicisms, and high-level multiplexing for rare mutation detection and single-cell gene expression analysis.
Dawne Shelton, PhD, Senior Scientist at the Digital Biology Center Tracking of cell-free tumor DNA during disease progression and treatment monitoring; early detection of tumor cfDNA in blood; detection of copy number amplifications; detection of KRAS mutations using multiplex screening
Dianna Maar, PhD, Senior Scientist at the Digital Biology Center Assay design guidelines; identification and resolution of assay design problems; assays design for detection of difficult targets; ddPCR using FFPE, cell-free DNA, and other fragmented or low-yield sample types
Jack Regan, PhD, Group Leader, Droplet Digital PCR Applications at the Digital Biology Center Discussion of how Droplet Digital PCR can be used to determine whether genomic sequences are physically linked, enabling us to assess disease carrier status, understand differences in allele-specific expression, and clarify genome architecture.