Wastewater-Based-Epidemiology (WBE) Monitoring with Droplet Digital™ PCR (ddPCR™) Technology

What is WBE and why is ddPCR technology such a good fit?

In recent years, public health officials have affirmed the need for robust methods capable of proactive detection of pathogens to alert to outbreaks and limit spread of disease. Longitudinal trend studies using wastewater-based epidemiology (WBE) and Droplet Digital™ PCR (ddPCR™) have been shown to be effective tools for these applications.

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The Past, Present, and Future of Wastewater-Based Epidemiology

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ddPCR Technology is An Ideal Tool for Wastewater-Based Epidemiology

Advantages of WBE:

  • Provides community-wide temporal trends
  • Near real-time data, depending on the analysis method
  • Passive sample collection that does not require individual participation approval

Advantages of ddPCR technology for WBE monitoring:

  • Fast analysis time
  • Absolute quantification
  • Sensitive enough to detect low copy numbers
  • Tolerant to PCR inhibitors that are present in wastewater

Application-Specific Resources

Wastewater-based epidemiology (WBE) and ddPCR technology are being used to address a broad set of health challenges, including SARS-CoV-2 (COVID), hMPXV, Influenza and RSV.

Additional Applications

Wastewater-based monitoring can test for other epidemiological pathogens. If it can be detected by PCR, wastewater monitoring with ddPCR technology could be applied.

The Bio-Rad and GT Molecular® Partnership

Both Bio-Rad and GT Molecular® have recognized the power and utility of wastewater-based epidemiology monitoring. Likewise, both have recognized the power and utility of ddPCR™ technology as the ideal detection method for WBE monitoring of human pathogens.

  • Over 10 year history of developing ddPCR instruments, assays, and consumables
  • Development of PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit
  • Development of ddPCR Expert Design Assays for monkeypox

  • Rapid response to hMPXV outbreak and collaboration with several wastewater facilities
  • Developer of testing services for wastewater parasites
  • Developed hMPXV kits optimized for the Bio-Rad QX200™ ddPCR™ system

Choose the best wastewater processing and testing solution

Our selection tool can help you determine which ddPCR™ solution best suits your unique research needs. Answer a few questions to find out!

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Ask a Bio-Rad specialist about ddPCR™ solutions that fit your need.

Access GT Molecular’s wastewater testing services: [email protected]

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Your selection: SARS-CoV-2 and/or hMPXV

Access GT Molecular kits, optimized for the Bio-Rad QX200 platform.

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Your selection: Influenza A/B, Norovirus, Adenovirus, RSV, Legionella, Enterococcus, or others.

Access Bio-Rad Expert Design Assays for these and other applications.

Access GT Molecular RSV kit, optimized for the Bio-Rad QX200 platform.

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We can now fully unlock the full potential of wastewater testing and surveillance as a public health tool with increased awareness, resources, and standardization.

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