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This poster describes how the HuCAL® antibody library and phage display technology were used to develop highly specific, anti-human EGF R and anti-human EGF R pTyr1045 antibodies for western blotting.
The antibody selection process was carried out in vitro providing greater flexibility for antibody generation compared with traditional animal-based immunization methods.
The best antibodies for total- and phospho-EGF R were tested side-by-side in western blotting under EGF and phosphatase treated conditions to show their different binding characteristics.
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