ddPCR, a new tool for fetal platelet genotyping from maternal plasma

Non invasive prenatal diagnosis for fetal platelet genotyping is a key for the diagnosis of HPA incompatibilities between the fetus and its mother and for managing fetal and neonatal alloimmunization thrombocytopenia. Non invasive prenatal diagnosis is based on fetal DNA detection in maternal plasma using different methodologies such as PCR-HRM, Cold-PCR, Next Generation Sequencing or ddPCR. After a long period of validation, the technology presently used in our lab is the ddPCR which unables us to give results to patients routinely and to be the only international laboratory able to determine 4 HPA incompatibilities simultaneously (HPA-1,-3,-5 and -15).

Rachel Petermann, MD,
In charge of Platelet Immunology Service at the Centre National de référence en hémobiologie périnatale (CNRHP) in Saint-Antoine Hospital