Best practices for designing a Western Blot experiment to detect phosphorylated proteins.
- Essentials for sample preparation
- Advice on selecting an antibody for detection of a phosphorylated protein
- Guidance on performing relative quantification analysis of a phosphorylated protein
- Tips to ensure robust data generation and cleaner blots
Mark Shulewitz, PhD,
Mark Shulewitz received his PhD in Molecular and Cell Biology from UC Berkeley followed by a post-doctoral fellowship in the Department of Molecular Oncology at Genentech. With over 20 years of R&D and Process Development experience in biotechnology focused on development and commercialization of therapeutic and research antibodies, Mark is currently an R&D Manager at Bio‑Rad.
Rachael Preston, PhD,
Rachael earned her PhD in cancer genetics at Cardiff University (UK) where she focused on characterizing the tumor suppression function of folliculin. Of particular focus was the detection of phosphorylation events resulting from the activation of kinases, like mTOR and AMPK. She has extensive experience in molecular biology techniques and antibody applications.
Peter Rettenberger, PhD,
Technical Services Supervisor
Peter has a Dr. rer. nat (PhD) from Technical University in Munich. As Business Unit Manager of a small biotechnology company, he managed the production of antibodies including phospho-specific antibodies. Peter is currently Technical Services Supervisor at the Oxford antibody division of Bio‑Rad offering global antibody product support.