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Antibody Guide

New Secondary Antibody Guide

Secondary Antibody Guide

Topics include:

  • Binding locations and CH2/3 domains
  • Schematic of immunoglobulin subclasses
  • Cross-adsorbed and isotype specific antibodies
  • Multiplexing
  • Antibody selection tool
  • Application protocols and resources

Your guide to successful secondary antibody experiments.

Download or Request Your Free Printed Copy

Bio-Rad’s secondary antibody guide is easy-to-read and practical, explaining step-by-step what you need to know, what you need to do, and how to do it. 
The guide is designed to help you find relevant information to optimize your experiments to get the right answers. 
Request a printed copy of the guide by submitting the form. We will fulfill requests while stock is available.


We loved that this entrant had focused on the AI search technology and ‘aid’, as in to help. With machine learning technology and the ability to help you find more with a single click.

FIN - (Find It Now)

This entry is an acronym for “Find It Now” but it is also French for “end”. We liked the association that you will end fruitless searches and easily and quickly find what you are looking for.


A cool and quirky name that is a play on “radar”. It highlights that our new search easily detects what you need.

Download or Request Your Free Printed Copy

Flow cytometry is an invaluable technology with the ability to generate a wide variety of biological data. However it is a complex technique which can be overwhelming particularly if you are at the beginning of your flow journey.

Our popular Flow Cytometry Basics Guide provides an essential tool to understand all the important facets of flow cytometry including how to optimize your experiments, what controls to use to get reliable data, and how to build multicolor panels.

You can download the full and/or pocket size of the guide below or request a printed copy by filling the form on this page.

To be informed when new resources like this are available, please sign up to receive our emailers.

New Secondary 
Antibody Guide

Flow Cytometry Basics Guide (Full edition)

The guide includes:

  • Principles of the Flow Cytometer
  • Principles of Fluorescence
  • Data Analysis
  • Controls in Flow Cytometry
  • Optimizing your Experiments
  • Multicolor Panel Building
  • Common Applications and New Technology
  • Common Protocols

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Sign up to receive our emailers by submitting the form on the right, to get updates on:

  • Free resources for your lab including application guides, posters, mini-reviews, protocols, and practical tips
  • Online tools
  • New product launches
  • Webinar invitations
  • Special offers
  • And more

We are constantly expanding our range of antibodies and reagents, resources, and online tools to help you choose the right antibodies, and design experiments with confidence so you can achieve more.

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Copy of the Guide:

Our Range

Bio-Rad offers over 3,000 flow cytometry tested antibodies available across many species with a range of 26 fluorophores as well as supporting reagents like viability dyes, Fc blocks, and staining buffers.

Find Your Antibody

Related Flow Cytometry Products

Other Resources

Viability Dye Fridge Magnet

Handy fridge magnet stating fixable and non-fixable viability dyes suitable for use in flow cytometry, and showing excitation and emission wavelengths.

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We have expanded the guide to include new sections on controls, experimental design, multicolor panel building, uses of flow cytometry and more.

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Designed as a quick reference guide when you are in the lab, this compact pocket guide contains checklists and tips to ensure your experiment will be a success.

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