Your guide to successful secondary antibody experiments.
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Flow cytometry is an invaluable technology with the ability to generate a wide variety of biological data. However it is a complex technique which can be overwhelming particularly if you are at the beginning of your flow journey.
Our popular Flow Cytometry Basics Guide provides an essential tool to understand all the important facets of flow cytometry including how to optimize your experiments, what controls to use to get reliable data, and how to build multicolor panels.
You can download the full and/or pocket size of the guide below or request a printed copy by filling the form on this page.
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The guide includes:
Bio-Rad offers over 3,000 flow cytometry tested antibodies available across many species with a range of 26 fluorophores as well as supporting reagents like viability dyes, Fc blocks, and staining buffers.
Find Your Antibody
Cell frequency can influence the choice of fluorophore and how many cells you will need to collect to obtain statistically significant data, crucial with rare populations and cell sorting. We have produced handy tables showing the relative frequency of cells in commonly used human, mouse and rat tissues, allowing you to plan and predict your flow populations.
The number of antigens or target molecules, that a cell carries, is important as it directly correlates to the intensity of the positive population and will determine the optimal fluorophore you should use for each marker. Knowing the antigen density will allow you to rank markers and pair them to similarly ranked fluorophores.
Use this guide to help you select the best fluorophore for your experiment.
Handy fridge magnet stating fixable and non-fixable viability dyes suitable for use in flow cytometry, and showing excitation and emission wavelengths.
This pocket version of our popular flow cytometry guide provides an overview of all of the important concepts in flow cytometry including: principles of the flow cytometer, fluorescence and fluorophores, data analysis, essential controls, gating process, optimizing your experiments, multicolor panel building and common protocols. This guide can be used as a quick reference when in the lab.