Bio-Rad Laboratories Logo

Western Blot Detection of IP
Samples - New Pocket Guide

Western Blot Detection of IP Samples - New Pocket Guide

Sign up to our emailers to receive a printed copy of our new handy pocket guide...


In an immunoprecipitation (IP) experiment, an antibody is cross-linked to agarose, sepharose, or magnetic beads to capture the protein of interest present in a lysate.

IP experiments and the detection of IP samples by western blotting can be challenging. Our new pocket guide contains a set of steps to help you with your experimental design.


Topics covered:


  • Select an immunoprecipitation (IP)
    tested antibody
  • Review of antibody binding affinities
  • Include appropriate controls
  • Carefully choose your lysis buffer
  • Concentrate your sample

Request your free IP pocket guide by completing the form.

Sign-up to receive a printed copy of our new handy pocket guide...


In an immunoprecipitation (IP) experiment, an antibody is cross-linked to agarose, sepharose, or magnetic beads to capture the protein of interest present in a lysate.

IP experiments and the detection of IP samples by western blotting can be challenging. Our new pocket guide contains a set of steps to help you with your experimental design.


Topics covered:


  • Select an immunoprecipitation (IP)
    tested antibody
  • Review of antibody binding affinities
  • Include appropriate controls
  • Carefully choose your lysis buffer
  • Concentrate your sample
  • Optimize the antibody amount
  • Wash efficiently
  • Select the best elution method
  • Denature your sample efficiently
  • Run an input sample on your western blot

Request your free IP pocket guide by completing the form.

  • Optimize the antibody amount
  • Wash efficiently
  • Select the best elution method
  • Denature your sample efficiently
  • Run an input sample on your western blot
Western Blot Detection of Immunoprecipitated Samples

Lineage Biomarkers of Human Immune System Guide


A guide that simplifies human marker selection by compiling all in one place:

  • Development of mature human immune cells from their precursors
  • 440 signature biomarkers (both CD and non-CD) specific to each immune cell type
  • Experimentally relevant information regarding each marker
  • Antibodies available for each marker

The booklet also includes a handy biomarker expression patterns grid enabling cell marker identification at a glance.



Biomarker Expression Patterns in Human Immune Cells Poster


This poster provides a cellular expression profile for 440 immune markers.

It is specifically designed to aid researchers in the quick and easy identification of more than 30 immune cell types.



Human Immune Cell Lineage & Antigen Expression Poster


A human lineage poster that details markers for each immune cell type to help you make this choice:

  • Simplifies marker selection
  • Easy to follow genealogical design
  • Color coded according to the exact cell types
  • Includes markers for myeloid cells, lymphocytes and stem cells

Working on Mouse Cells?


Find your cell specific markers using our mouse immune cell lineage and expression guide, posters and interactive database.


Sign up to receive our emailers and get a printed IP pocket guide.

Lineage Biomarkers of Human Immune System Guide


A guide that simplifies human marker selection by compiling all in one place:


  • Development of mature human immune cells from their precursors
  • 440 signature biomarkers (both CD and non-CD) specific to each immune cell type
  • Experimentally relevant information regarding each marker
  • Antibodies available for each marker

The booklet also includes a handy biomarker expression patterns grid enabling cell marker identification at a glance.

Biomarker Expression Patterns in Human Immune Cells Poster


This poster provides a cellular expression profile for 440 immune markers.


It is specifically designed to aid researchers in the quick and easy identification of more than 30 immune cell types.


Human Immune Cell Lineage & Antigen Expression Poster


A human lineage poster that details markers for each immune cell type to help you make this choice:


  • Simplifies marker selection
  • Easy to follow genealogical design
  • Color coded according to the exact cell types
  • Includes markers for myeloid cells, lymphocytes and stem cells
Western Blot Detection of Immunoprecipitated Samples
Copyright © Bio-Rad Laboratories, Inc. | | All rights reserved. | Trademarks | Privacy Policy