Western Blot Detection of
IP Samples Guide

Download or Request Your Free Printed Copy

In an immunoprecipitation (IP) experiment, an antibody is cross-linked to agarose, sepharose, or magnetic beads to capture the protein of interest present in a lysate.

IP experiments and the detection of IP samples by western blotting can be challenging. Our pocket guide contains a set of steps to help you with your experimental design.

Request your free IP pocket guide by completing the form.

Western Blot Detection of IP Samples - Pocket Guide

Topics covered:

  • Select an immunoprecipitation (IP)
    tested antibody
  • Review of antibody binding affinities
  • Include appropriate controls
  • Carefully choose your lysis buffer
  • Concentrate your sample
  • Optimize the antibody amount
  • Wash efficiently
  • Select the best elution method
  • Denature your sample efficiently
  • Run an input sample on your western blot

Receive updates on resources like this straight into your inbox...

Sign up to receive our emailers by submitting the form on the right, to get updates on:

  • Free resources for your lab including application guides, posters, mini-reviews, protocols, and practical tips
  • Online tools
  • New product launches
  • Webinar invitations
  • Special offers
  • And more
Receive updates on resources

We are constantly expanding our range of antibodies and reagents, resources, and online tools to help you choose the right antibodies, and design experiments with confidence so you can achieve more.

Follow Us

facebook icon
twitter icon
linkedin icon
youtube icon
pinterest icon
RSS icon
wechat icon

Request a Printed Copy of the Guide:

Thank you