In an immunoprecipitation (IP) experiment, an antibody is cross-linked to agarose, sepharose, or magnetic beads to capture the protein of interest present in a lysate.
IP experiments and the detection of IP samples by western blotting can be challenging. Our pocket guide contains a set of steps to help you with your experimental design.
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Western Blot Detection of IP Samples - Pocket Guide
- Select an immunoprecipitation (IP)
- Review of antibody binding affinities
- Include appropriate controls
- Carefully choose your lysis buffer
- Concentrate your sample
- Optimize the antibody amount
- Wash efficiently
- Select the best elution method
- Denature your sample efficiently
- Run an input sample on your western blot
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