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Poster: Anti-Biotherapeutic Antibody Development against Ipilimumab with HuCAL PLATINUM®

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Poster: Anti-Biotherapeutic Antibody Development against Ipilimumab with HuCAL PLATINUM®

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This poster presents the typical workflow for Bio-Rad's anti-idiotypic antibody projects using generation and characterization of antibodies against the checkpoint inhibitor drug ipilimumab (YERVOY) as an example.

Antibodies in monovalent Fab format were characterized for specificity, affinity, and inhibitory potential, and were screened for their ability to work in a pharmacokinetic bridging ELISA for drug quantification. Selected Fab antibodies were converted into full length, fully human IgG1 format and evaluated in an anti-drug-antibody bridging ELISA.

Inhibitory antibodies that recognize the free drug, and antibodies that recognize the drug-target complex were developed and are available in our catalog.
Anti-Biotherapeutic Antibody Development against Ipilimumab with HuCAL PLATINUM® Poster
Epidermal Growth Factor Receptor Pathway Poster

mTOR Signaling Poster


Understanding mTOR signaling provides a basis for developing mTOR inhibitors, and inhibitors of components within the wider signaling pathway. Inhibition of the mTOR pathway is relevant for cancer, obesity, diabetes, and even aging.

Uncover the proteins involved in our poster.


Your guide to successful secondary antibody experiments.

AIDEN

We loved that this entrant had focused on the AI search technology and ‘aid’, as in to help. With machine learning technology and the ability to help you find more with a single click.

FIN - (Find It Now)

This entry is an acronym for “Find It Now” but it is also French for “end”. We liked the association that you will end fruitless searches and easily and quickly find what you are looking for.

RAY DAR

A cool and quirky name that is a play on “radar”. It highlights that our new search easily detects what you need.

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Flow cytometry is an invaluable technology with the ability to generate a wide variety of biological data. However it is a complex technique which can be overwhelming particularly if you are at the beginning of your flow journey.

Our popular Flow Cytometry Basics Guide provides an essential tool to understand all the important facets of flow cytometry including how to optimize your experiments, what controls to use to get reliable data, and how to build multicolor panels.


You can download the full and/or pocket size of the guide below or request a printed copy by filling the form on this page.

To be informed when new resources like this are available, please sign up to receive our emailers.

Flow Cytometry Basics Guide (Full edition)


The guide includes:


  • Principles of the Flow Cytometer
  • Principles of Fluorescence
  • Data Analysis
  • Controls in Flow Cytometry
  • Optimizing your Experiments
  • Multicolor Panel Building
  • Common Applications and New Technology
  • Common Protocols
KEEP AN EYE OUT

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We will be launching new posters focussing on more signaling pathways very soon, sign up for our email communications to stay updated...


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We are constantly expanding our range of antibodies and reagents, resources, and online tools to help you choose the right antibodies, and design experiments with confidence so you can achieve more.


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  • Application notes
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  • Updates on new anti-biotherapeutic antibodies launched
  • New custom services available

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Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. HuCAL and HuCAL PLATINUM are trademarks of MorphoSys AG. All trademarks used herein are the property of their respective owner.

Our Range

Bio-Rad offers over 3,000 flow cytometry tested antibodies available across many species with a range of 26 fluorophores as well as supporting reagents like viability dyes, Fc blocks, and staining buffers.


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Viability Dye Fridge Magnet

Handy fridge magnet stating fixable and non-fixable viability dyes suitable for use in flow cytometry, and showing excitation and emission wavelengths.




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We have expanded the guide to include new sections on controls, experimental design, multicolor panel building, uses of flow cytometry and more.


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NEW EDITION



Designed as a quick reference guide when you are in the lab, this compact pocket guide contains checklists and tips to ensure your experiment will be a success.


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Schedule a call with a technical specialist by filling the form
NF-κB pathway
Poster

NF-kB Signaling Poster


Nuclear Factor-Kappa B is a transcription factor responsible for generating the protective inflammatory response following infection or injury. However, constitutive activation of NF-κB leads to chronic diseases and inflammatory disorders, and promotes cancer progression.

Understanding the balance between the roles of NF-κB in cell health and disease is fundamental in the development of targeted inhibitors of inflammation. Uncover the proteins involved.


Epidermal Growth Factor Receptor Pathway Poster

Epidermal Growth Factor Receptor Signaling Poster


ErbB family members are instrumental in the development and malignancy of various human cancers.

Visualize the key proteins involved in the EGF R signaling pathways and how they regulate diverse biological functions, like cell proliferation, differentiation, migration, and apoptosis, with our poster.


View related EGFR antibodies
View related mTOR antibodies

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