Recent progress in the field of Genome Editing has opened new applications and allowed researchers to generate new animal models to study gene function and diseases. Genome Editing has traditionally been hampered poor efficacy and labor-intensive procedures. Electroporation based solution significantly mitigate this challenge. On top of that, it has been cited (Chen S et al 2016) as a simple and economical strategy especially for in vivo genome editing.
Another major challenge to Genome Editing is that isolating rare recombinant iPS cell clones is difficult without antibiotic selection. To help solve this problem, Dr. Yuichiro Miyaoka and his team has developed a method (Miyaoka et al 2014) that allows efficient clone selection.
|DATE:||9th August 2017, Wednesday|
|TIME:||10:00 AM to 12.30 PM|
Kenneth Lo Classroom (P4703) (map)
4/F Yeung Kin Man Academic Building (Purple Zone)
|HOST:||City University of Hong Kong
- Department of Biomedical Science
|Yuichiro MIYAOKA, Ph.D.
Tokyo Metropolitan Institute of Medical Science,
Welcome & Registration
Molecular Applications of Droplet Digital PCR™
Alfred CHAN, Ph.D.
Detection of Genome Editing Outcomes
Yuichiro MIYAOKA, Ph.D.
Improving CRISPR Genome Editing using Hi-Fidelity Cas9 protein and chemically modified crRNAs as an RNP complex
Michel CANNIEUX, Ph.D., MBA
Registration closes: 7th August 2017.
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