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Bio-Rad is dedicated to offering tools and services that empower you in your development of cutting edge gene and cell therapies.

Driven by the desire to be your partner in the industry, we are here to support your product stability and expression, protein purification, antibody development and selection, cell phenotyping, on/off target effects, and predictive biomarker applications. Understanding the importance of quality in manufacturing we offer platforms to simplify your workflow for viral titer, vector copy number, residual DNA, and protein expression profiling.

Utilize this site for articles, tips & tricks, protocols, and resources to support your gene and cell therapy development.






Discovery & Development





Product Stability & Expression

Protein Stability & Expression

Limited speed and throughput can be challenging when measuring protein stability and expression.

Our Western workflow can increase throughput compared to traditional blotting by allowing you to complete electrophoresis and transfer in less than 30 minutes. In addition, increase confidence in your Western blots by eliminating the use of unreliable house-keeping proteins with Total Protein Normalization using our Stain-Free workflow.

The ChemiDoc Imaging System is a single solution for all of your gel imaging and blotting applications including DNA and protein stains, as well as chemiluminescence and fluorescence Western blots.



Interested in learning more? Speak with our proteomics sales specialist ▸




Confirm Transgene Expression

Verifying transgene expression is an essential but time-consuming process due to the large number of transcripts and samples that are often analyzed. Our CFX Real-Time and automation solutions enable scalable qPCR while minimizing plate processing time.



Parallel Analysis of RNA and Protein Expression: A Superior Method
Differential Anti-Apoptotic Gene Expression Patterns: A Semi-Automated Quantitative Approach



Interested in learning more? Speak with our genomics sales specialist ▸







Protein Purification

Method development and resin selection can be two of the biggest challenges in your protein purification workflow. A flexible system that can be easily automated and has built in methods for optimization shortens the time to results ensuring your project stays on track.

The NGC chromatography system has the ability to purify large complex biomolecules such as viruses and antibodies, oligos, peptides.



Bio-Rad also offers scalable resins that are suitable for manufacturing.

  • Ceramic hydroxyapatite (CHT)
  • UNOsphereQ
  • Nuvia Q



Talk to a proteomics sales specialist ▸







Antibody Development and Selection for Cell Therapy

CAR and engineered T-cell receptor (TCR) therapy research and development requires highly specialized antibody reagents. Developing antibodies for these therapies can be very challenging. Specificity against the CAR or TCR is imperative to success, and time is of the essence.

Advantages of using Bio-Rad’s custom HuCAL monoclonal antibody generation services:

  • Highly specific antibodies detecting the CAR or engineered T-cell
  • Guided selection allowing for specific binding and blocking
  • Affinity maturation to ensure the highest possible binding
  • Antibody sequence available for security of supply and independence
  • Antibodies available in as little as 8 weeks

Custom Recombinant Monoclonal Antibody Generation: HuCAL Technology Explained



Anti-Idiotypic Antibodies for Development of CAR T Cell Therapies and Monoclonal Antibody Drugs
 
Designed Just for You — Discover the Technology Behind
Bio-Rad's Custom Recombinant Antibodies
 



Chat with a HuCAL sales specialist ▸







Cell Phenotyping

Characterizing cell types and cellular behavior involved in the immune responses to cancer and identifying which cells make the best targets for therapies are of paramount importance for the creation of engineered, therapeutic cells. At the same time, the signatures that characterize these discrete cellular populations are becoming more complex. These multiplex, large-scale assays can therefore benefit from automated high-throughput, high-parameter flow cytometry in order to identify cells faster.

The ZE5 Cell Analyzer provides the speed, flexibility, and high-order multiplexing, all while offering maximal productivity with integrated automation and 24/7 operation.

High-throughput flow cytometry: Speed up your cancer biomarker discovery































Speak with a flow cytometry sales specialist ▸







On/Off Target Effects

When examining immune responses, collecting and analyzing data from multiple targets is slow and tedious. In addition, limited sample can pose challenges if you want to measure multiple targets.

Bio-Plex multiplex solution can help by saving time, sample, and resources compared to traditional ELISA:

  • Measure multiple cytokines and chemokines in a single plate
  • Analyze human and mouse with different sample types such as serum and plasma
  • Utilize an easy to use operation and analysis software package
  • Collect and quickly analyze data



Interested in learning more? Speak with our proteomics sales specialist ▸







Predictive Biomarker Discovery

Identifying patients who will most likely benefit from immunotherapy increases the probability of a successful clinical trial and avoids unnecessary toxicity and health care costs. However, the search for reliable predictive biomarkers is limited by our incomplete understanding of the complex immune response elicited by these therapies. Bio-Rad offers multiple solutions to help streamline the screening process of potential biomarker targets.







Questions? We can help! ▸
 
Questions? We can help! ▸

GENE AND CELL THERAPY

Your partner from discovery to therapeutic


#init4thecure









Analytical Development & QC



Droplet Digital PCR

Droplet Digital PCR (ddPCR) provides highly precise absolute quantification of nucleic acid sequences. ddPCR measures absolute quantities by counting nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplet partitions. Bio-Rad’s ddPCR systems utilize a simple workflow with minimal hands-on time, while reducing sample replicates and user-to-user variability.


How Digital PCR is used in Drug Discovery and Development
 


The QX200 ddPCR system support is designed to meet manufacturing and QC environment needs:

  • Regulatory edition software to assist with 21 CFR Part 11 compliance
  • Installation qualification/operational qualification (IQ/OQ) service
  • Premier service offerings with rapid on-site support


Speak with a ddPCR expert ▸




Viral Titer

Exact viral titration of vector genome copies is critical for ensuring accurate and reproducible dosing in both preclinical and clinical settings. It is equally important for optimal transduction efficiency and downstream potency of cell therapies. Droplet digital PCR (ddPCR) directly quantifies DNA copies with exemplary precision without a standard curve.







Vector Copy Number

Accurate vector copy number is important when trying to achieve an ideal therapeutic dose. The precision of ddPCR enables detection of small fold copy number changes providing confidence in discriminating 3 vs 4 integration events.







Residual DNA

Residual DNA content is common cell and gene therapy manufacturing impurity and levels must be carefully monitored for lot release. ddPCR is a precise and sensitive method for direct quantitation of residual DNA content. Since ddPCR does not rely on reaction efficiency for quantitation, it is less prone to sample interference from complex matrices and process intermediates.










Protein Expression Profiling

Measuring integrity, quantity, and identity of proteins is a key element of your manufacturing process. Reproducibility, sensitivity, and automation are key to your success.

The Biologics Analysis Workflow is a suite of products designed and validated to assess the purity of proteins in a GMP environment.

  • Save time by increasing the speed of electrophoresis to 20 min or less
  • Increase sensitivity using QC colloidal Coomassie stain
  • Enhance reproducibility and accuracy with the GS-900 calibrated densitometer
  • Automated analysis using ImageLab software that does not require user expertise and is CFR 21 part 11 compliant

Biologics Analysis: A Workflow Overview

If part of your process also includes protein identity using Western blot, the ChemiDoc MP is designed to meet your needs.

  • Advanced blot detection ensuring the optimal image and sensitivity
  • Automated analysis using ImageLab software, the same software as the GS-900 calibrated densitometer with CFR 21 part 11 compliance
  • Installation qualification/operational qualification (IQ/OQ) available

ChemiDoc MP: Fluorescence and chemiluminescence detection without compromises



Speak with a sales specialist ▸




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