Learn Flow Your Way with Bio‑Rad’s Resources
Flow cytometry is an invaluable technology with the ability to generate a wide variety of biological data. However it is a complex technique which can be overwhelming particularly if you are at the beginning of your flow journey.
To support you, we have compiled some useful resources including our popular Flow Cytometry Basics Guide, covering key topics such as how to optimize your experiments, what controls to use to get reliable data, and how to build multicolor panels.
UPDATED Flow Cytometry Basics GuideFlow Cytometry - The Essentials
Fluorescent Dyes Poster
Flow Cytometry Poster
To help you plan your experiments, you may also be interested in our fluorophore reference guide and Steps to Success posters.
These resources are available to download now. You can also request free printed copies by submitting the form overleaf.
UPDATED
Flow Cytometry Basics Guide
Topics covered in the guide include:
- Principles of the Flow Cytometer
- Principles of Fluorescence
- Data Analysis
- Controls in Flow Cytometry
- Optimizing your Experiments
- Multicolor Panel Building
- Common Applications and New Technology
- Common Protocols
- Troubleshooting
UPDATED
The new, updated Flow Cytometry Basics Guide has it all covered. From an introduction to basic flow cytometry principles, practical advice for planning and optimizing your experiments, to examples of conventional and spectral multicolor panels. Ideal for beginners and for use as a teaching aide.
Download the GuideFlow Cytometry - The Essentials (Pocket Guide Version)
The guide includes:
- Know your Cytometer
- Understanding Fluorescence and Fluorophores
- Gating Process
- Controls
- Optimization
- Panel Building
- Simple Staining Protocols
- Flow Cytometry Resources
Designed as a quick reference guide when you are in the lab, this compact pocket guide contains checklists and tips to ensure your experiment will be a success.
Download the GuideFluorescent Dyes Poster
Essentials for Multicolor Panel Building
On this free poster, you can find essential information for common fluorescent dyes including optimal laser excitation, maximal emission and relative brightness, as well as handy tips for panel design.
Perfect as a reference guide to check dyes' compatibility before you start your experiments.
Designed as a quick reference guide when you are in the lab, this compact pocket guide contains checklists and tips to ensure your experiment will be a success.
Download the PosterFlow Cytometry Poster
Planning your flow cytometry experiment can be time consuming. With this in mind, this poster aims to help you plan your experiment by going through the key steps you need to consider before you start.
It also includes information on staining protocols and useful tips to optimize your staining.
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