Flow Cytometry

Bio-Rad, the place to go for flow

Learn Flow Your Way with Bio‑Rad’s Resources

Flow cytometry is an invaluable technology with the ability to generate a wide variety of biological data. However it is a complex technique which can be overwhelming particularly if you are at the beginning of your flow journey.

To support you, we have compiled some useful resources including our popular Flow Cytometry Basics Guide, covering key topics such as how to optimize your experiments, what controls to use to get reliable data, and how to build multicolor panels.

To help you plan your experiments, you may also be interested in our fluorophore reference guide and Steps to Success posters.

These resources are available to download now. You can also request free printed copies by submitting the form overleaf.

Flow Cytometry Basics Guide
(Full edition — online only)

The guide includes:

  • Principles of the Flow Cytometer
  • Principles of Fluorescence
  • Data Analysis
  • Controls in Flow Cytometry
  • Optimizing your Experiments
  • Multicolor Panel Building
  • Common Applications and New Technology
  • Common Protocols
Guide Download Only

Designed as a reference for all flow users, our popular flow cytometry guide contains a basic overview of all the important facets of flow cytometry.

Download the Guide 

Flow Cytometry - The Essentials (Pocket Guide Version)

The guide includes:

  • Know your Cytometer
  • Understanding Fluorescence and Fluorophores
  • Gating Process
  • Controls
  • Optimization
  • Panel Building
  • Simple Staining Protocols
  • Flow Cytometry Resources

Designed as a quick reference guide when you are in the lab, this compact pocket guide contains checklists and tips to ensure your experiment will be a success.

Download the Guide 

Fluorophore Poster

Essentials for Multicolor Panel Building

On this free poster, you can find essential information for common flow cytometry fluorophores including optimal laser excitation, maximal emission and relative brightness, as well as handy tips for panel design.

Perfect as a reference guide to check fluorophore compatibility before you start your experiments.


Designed as a quick reference guide when you are in the lab, this compact pocket guide contains checklists and tips to ensure your experiment will be a success.

Download the Poster 

Flow Cytometry Poster

Planning your flow cytometry experiment can be time consuming. With this in mind, this poster aims to help you plan your experiment by going through the key steps you need to consider before you start.

It also includes information on staining protocols and useful tips to optimize your staining.

brodchure Download Only

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We are constantly expanding our range of antibodies and reagents, resources, and online tools to help you choose the right antibodies, and design experiments with confidence so you can achieve more.

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Bio‑Rad offers over 3,000 flow cytometry tested antibodies available across many species with a range of 26 fluorophores as well as supporting reagents like viability dyes, Fc blocks, and staining buffers.

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Request a Printed Copy of the Guide(s) or Poster(s):

Thank you

Thank you for your order of printed copies of our Flow Cytometry Resources which will be sent to you within the next few weeks.