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Flow Cytometry

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Learn Flow Your Way with Bio-Rad’s Resources

Flow cytometry is an invaluable technology with the ability to generate a wide variety of biological data. However it is a complex technique which can be overwhelming particularly if you are at the beginning of your flow journey.

To support you, we have compiled some useful resources including our popular Flow Cytometry Basics Guide, covering key topics such as how to optimize your experiments, what controls to use to get reliable data, and how to build multicolor panels.

To help you plan your experiments, you may also be interested in our fluorophore reference guide and Steps to Success posters.


These resources are available to download now. You can also request free printed copies by submitting the form overleaf.

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Download or Request Your Free Printed Copy

Flow cytometry is an invaluable technology with the ability to generate a wide variety of biological data. However it is a complex technique which can be overwhelming particularly if you are at the beginning of your flow journey.

Our popular Flow Cytometry Basics Guide provides an essential tool to understand all the important facets of flow cytometry including how to optimize your experiments, what controls to use to get reliable data, and how to build multicolor panels.


You can download the full and/or pocket size of the guide below or request a printed copy by filling the form on this page.

To be informed when new resources like this are available, please sign up to receive our emailers.

Flow Cytometry Basics Guide (Full edition)


The guide includes:


  • Principles of the Flow Cytometer
  • Principles of Fluorescence
  • Data Analysis
  • Controls in Flow Cytometry
  • Optimizing your Experiments
  • Multicolor Panel Building
  • Common Applications and New Technology
  • Common Protocols

Flow Cytometry Basics Guide (Full edition)


The guide includes:


  • Principles of the Flow Cytometer
  • Principles of Fluorescence
  • Data Analysis
  • Controls in Flow Cytometry
  • Optimizing your Experiments
  • Multicolor Panel Building
  • Common Applications and New Technology
  • Common Protocols

Flow Cytometry - The Essentials (Pocket Guide Version)


The guide includes:


  • Know your Cytometer
  • Understanding Fluorescence and Fluorophores
  • Gating Process
  • Controls
  • Optimization
  • Panel Building
  • Simple Staining Protocols
  • Flow Cytometry Resources

Request a Printed Copy of the Guide(s) or Poster(s):

Our Range

Bio-Rad offers over 3,000 flow cytometry tested antibodies available across many species with a range of 26 fluorophores as well as supporting reagents like viability dyes, Fc blocks, and staining buffers.


Find Your Antibody

Related Flow Cytometry Products

Other Resources

Viability Dye Fridge Magnet

Handy fridge magnet stating fixable and non-fixable viability dyes suitable for use in flow cytometry, and showing excitation and emission wavelengths.




Our Range

Bio-Rad offers over 3,000 flow cytometry tested antibodies available across many species with a range of 26 fluorophores as well as supporting reagents like viability dyes, Fc blocks, and staining buffers.


Find Your Antibody


We have expanded the guide to include new sections on controls, experimental design, multicolor panel building, uses of flow cytometry and more.


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NEW EDITION



Designed as a quick reference guide when you are in the lab, this compact pocket guide contains checklists and tips to ensure your experiment will be a success.



Designed as a reference for all flow users, our popular flow cytometry guide contains a basic overview of all the important facets of flow cytometry.


Fluorophore Poster

Essentials for Multicolor Panel Building

On this free poster, you can find essential information for common flow cytometry fluorophores including optimal laser excitation, maximal emission and relative brightness, as well as handy tips for panel design.


Perfect as a reference guide to check fluorophore compatibility before you start your experiments.


Flow Cytometry Poster


Planning your flow cytometry experiment can be time consuming. With this in mind, this poster aims to help you plan your experiment by going through the key steps you need to consider before you start.

It also includes information on staining protocols and useful tips to optimize your staining.


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