Learn how to design no compensation panels
The presentation by Dr. Sharon Sanderson will be supplemented by English, Mandarin (Simplified Chinese) and Japanese subtitles, followed by a live Q&A.
英語／日本語／中国語字幕付き
Advances in technology and increased fluorescent dye choice means there are more pitfalls to navigate during panel design. However, some simple steps can be followed to make this less daunting and help you get the right data first time. Often biological questions can be answered with low plex panels where careful design can avoid compensation steps, making experiments simpler and quicker.
In this webinar, we describe a seven-step process for panel design that has been followed to generate human and mouse no/low compensation immunophenotyping panels. These panels generate data of a high quality, but with simplified acquisition and analysis. They also work as a backbone framework to build higher plex panels and save on reagents and time. We will show you how you can build your own no/low compensation panels for conventional instruments, that will also work for spectral systems too, enabling you to generate reliable and reproducible results.
You will learn:
- The benefits of no/low compensation panels
- Seven steps for successful panel design
- How to apply these steps to the design of mouse and human no/low compensation panels for conventional cytometers
- How to use no/low compensation panels as backbones for larger panels
- Why StarBright™ Dyes are ideal for including in multiplexing panels
Dr. Sharon Sanderson,
Flow Cytometry Applications Scientist and Product Manager at Bio-Rad
Sharon Sanderson, PhD, is a Flow Cytometry Applications Scientist and Product Manager at Bio-Rad. She obtained her PhD in Cell Biology from the Institute of Cancer Research with her thesis focusing on cell signaling pathways in angiogenesis. Sharon then moved to The University of Oxford where she worked for 13 years. She initially continued to study the field of angiogenesis before moving into immunology research.