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Practical advice you can trust, use Bio-Rad for ELISA

ELISA Guides

New Secondary
Antibody Guide

Topics include:

  • Binding locations and CH2/3 domains
  • Schematic of immunoglobulin subclasses
  • Cross-adsorbed and isotype specific antibodies
  • Multiplexing
  • Antibody selection tool
  • Application protocols and resources

Your guide to successful secondary antibody experiments.

Bio-Rad’s Antibody Product Range and Resources at a Glance

Download or Request Free Printed ELISA Resources

ELISA is the most frequently used application in all fields of life science research. It is the first assay applied when antibodies are characterized and it is relevant to an immense variety of analyte assays from pure research to clinical diagnostics.

ELISAs are widely applied in immune research and diagnostic applications. Success of your ELISAs will depend on following key steps when planning experiments. This is where Bio-Rad’s application guides can help.

An Introduction to ELISA - Basics Guide
(Full edition)

The ELISA Basics Guide is a thorough reference for all users of this multi-faceted procedure. This guide explains how and why ELISA has developed since the 1970s and the methods required to optimize your experiments.

The guide includes:

  • ELISA Technology
  • ELISA Detection Options
  • Controls
  • ELISA Optimization
  • Protocols
  • ELISA Troubleshooting


We loved that this entrant had focused on the AI search technology and ‘aid’, as in to help. With machine learning technology and the ability to help you find more with a single click.

FIN - (Find It Now)

This entry is an acronym for “Find It Now” but it is also French for “end”. We liked the association that you will end fruitless searches and easily and quickly find what you are looking for.


A cool and quirky name that is a play on “radar”. It highlights that our new search easily detects what you need.

Download or Request Your Free Printed Copy

Flow cytometry is an invaluable technology with the ability to generate a wide variety of biological data. However it is a complex technique which can be overwhelming particularly if you are at the beginning of your flow journey.

Our popular Flow Cytometry Basics Guide provides an essential tool to understand all the important facets of flow cytometry including how to optimize your experiments, what controls to use to get reliable data, and how to build multicolor panels.

You can download the full and/or pocket size of the guide below or request a printed copy by filling the form on this page.

To be informed when new resources like this are available, please sign up to receive our emailers.

New Overview Poster

Flow Cytometry Basics Guide (Full edition)

The guide includes:

  • Principles of the Flow Cytometer
  • Principles of Fluorescence
  • Data Analysis
  • Controls in Flow Cytometry
  • Optimizing your Experiments
  • Multicolor Panel Building
  • Common Applications and New Technology
  • Common Protocols

ELISA - The Essentials Pocket Guide

The Essentials Pocket Guide is a condensed version of our ELISA - Basics Guide, focusing on key facts about ELISA design and execution. It is just the right size to keep with you in your lab coat pocket, ready for immediate consultation.

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We are constantly expanding our range of antibodies and reagents, resources, and online tools to help you choose the right antibodies, and design experiments with confidence so you can achieve more.

Request a Copy of
the Printed Guide(s)

Our Range

Bio-Rad offers over 3,000 flow cytometry tested antibodies available across many species with a range of 26 fluorophores as well as supporting reagents like viability dyes, Fc blocks, and staining buffers.

Find Your Antibody

Related Flow Cytometry Products

Other Resources

Viability Dye Fridge Magnet

Handy fridge magnet stating fixable and non-fixable viability dyes suitable for use in flow cytometry, and showing excitation and emission wavelengths.

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We have expanded the guide to include new sections on controls, experimental design, multicolor panel building, uses of flow cytometry and more.

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Designed as a quick reference guide when you are in the lab, this compact pocket guide contains checklists and tips to ensure your experiment will be a success.

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The ELISA Basics Guide is a thorough reference for all users of this multi-faceted procedure. This guide explains how and why ELISA has developed since the 1970s and the methods required to optimize your experiments.

Our Range

Bio-Rad offers a wide range of antibodies tested in ELISA, kits, buffers, assay diluents, and substrates.

View the Full Range

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