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Discover Bio-Rad at

ASHG 2019 Annual Meeting

Booth #530 | Houston, TX | Oct. 15-19

Visit us at booth #530 and learn about the SEQuoia RNA-Seq Kit, ddPCR, the S3e Cell Sorter and the ddSEQ Single-Cell Isolator

Thursday, October 17 Agenda

12:45PM - 1:15PM

Single‑cell genome-wide profiling of the epigenomic landscape using Bio‑Rad's scATAC‑Seq solution

Bio-Rad Laboratories Exhibitor Education Event

Speaker: Ron Lebofsky

Ron Lebofsky,
Digital Biology Division, Bio‑Rad Laboratories Inc.

Marriott Marquis Houston – Meyerland AB, Level 3


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1:20PM - 2:00PM

Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility

Bio-Rad Laboratories Exhibitor Education Event

Speaker: Caleb Lareau

Caleb Lareau,
Harvard University

Marriott Marquis Houston – Meyerland AB, Level 3


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2:00PM - 3:00PM

Simultaneous capture of short and long RNA subtypes from liquid biopsy substrates and FFPE tissue samples by a novel RNA-Seq library prep method

Poster Session

Technological advances in recent years have accelerated the study of biological markers as diagnostic, prognostic, or predictive indicators of a physiological state. Through the use of RNA sequencing (RNA-Seq) technology, researchers are querying the differential expression of messenger RNA (mRNA) transcripts and non-coding RNAs (lncRNA) as potential biomarkers of disease. One limitation that impedes discovery is the inability to query all RNA subtypes in a sample simultaneously. Most commercially available RNA-Seq library preparation methods are limited in their ability to capture only long or short RNAs. Here, we describe a new library preparation kit that utilizes a novel enzyme to capture all RNA subtypes at great sensitivity and independently of their size, enabling the simultaneous differential expression analysis of mRNA, lncRNA, miRNA, snoRNA, and other subtypes. Furthermore, we demonstrate the robustness of the enzyme and its ability to capture RNA from complex and highly degraded biological samples, such as liquid biopsies substrates and FFPE tissue samples.

Authors:
S. Okino 1; A. Bibillo 1; P. Patel 1; Y. Wang 1; L. Lingelbach 2; Y. Jouvenot 2

Convention Center – Exhibit Hall, Level 1


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2:00PM - 3:00PM

Screening Alzheimer Disease Serum for Long Noncoding RNA Biomarkers

Poster Session

Long noncoding RNAs have become the fastest growing class of biomarkers in genomics research. They have been demonstrated to play roles in normal development mechanisms as well as pathological processes, such as cancer, autoimmune diseases and neurodegenerative diseases. In order to find new and relevant biomarkers for Alzheimer’s Disease, we designed an approach to quantify changes in expression of long noncoding RNA targets from patient serum. We purified cell free RNA from a portion of each serum sample, and found that the majority of samples did have low amounts of RNA, although a few were too degraded for further analysis. Using a one-step pre-amplification reverse transcription qPCR strategy, we screened for differential expression of long non coding RNAs proposed to be associated with inflammation and epigenetics. Alzheimer’s Disease is proposed to be a disease of inflammation, and disease states are often the result of epigenetic dysregulation. Of the 145 unique long noncoding RNAs screened, we identified four with a greater than two fold expression change over healthy controls that were deemed significant (p<0.05) by ANOVA, and had normal sample distributions by the Shapiro-Wilks normality test. All four of these lncRNAs (AC093323.3, BDNF-AS, H19 and HAR1B) are linked to epigenetic processes.

Authors:
E. Dreskin; M. Hammond; R. Drevland; Y. Jouvenot

Convention Center – Exhibit Hall, Level 1


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3:15PM - 4:00PM

Laboratory CoLab: Development of RNA-seq based Endovascular Biopsy for Brain Arteriovenous Malformations

A novel biopsy technique with complete transcriptome analysis capabilities performed on rare, neuronal-derived cell populations via comprehensive RNA-seq library preparation with a new molecular platform.

Ethan A. Winkler, M.D., Ph.D. Resident Physician Department of Neurological Surgery University of California San Francisco

Pranav Patel, Ph.D. Head of Sequencing Assays Bio-Rad Laboratories

Convention Center – Exhibit Hall, CoLab Theater 1 #345


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