The ProteOn Label-Free Webinars feature presentations from thought leaders in the field of label-free biomolecular interaction analysis using surface plasmon resonance (SPR). In the first series of webinars this year, Dr. Olan Dolezal will kick us off with his presentation, "Lead discovery: Screening and characterization using multiplexed SPR." Next,Dr. Emiliano Biasini will present "Identification of novel prion protein-based compounds to block the toxicity of Alzheimer's Aβ oligomers." Finally, Dr. Jay Duffner will share "Novel applications of histidine-tag capture using the ProteOn XPR36 system." You may register for one or all of the webinars. Registration also gives you access to our extensive library of past SPR webinars. Please click below to sign up.

2013 ProteOn Webinar Series

  • april 23rd

    1. Lead discovery: Screening and characterization using multiplexed SPR

    For over twenty years Surface Plasmon Resonance (SPR) instruments have been utilized to characterize biomolecular interactions and in so doing provide valuable data for the selection of candidate molecules with therapeutic potential. With the improved instrumental throughput and development of collective 'know-how', SPR technology has become an essential tool applicable throughout the entire drug discovery process. Fragment-based lead discovery (fragment screening) has been increasingly used for finding lead compounds as part of small molecule drug discovery process. It is based on identifying small chemical fragments, which may bind only weakly to the biological target, and then combining or 'growing' them to produce leads with higher affinities. For the fragment screening, analysis of multiple interactions in parallel using multiplexed SPR (ProteOn XPR36) offers several advantages over conventional SPR including increased productivity and performance, and ability to conduct rapid comparisons of different experimental conditions.

    Presenter
    Olan Dolezal, PhD
    Senior Research Scientist
    CSIRO
    Australia

    April 23, 2013
    Europe: 9 A.M. Central Europe Time
    Pacific: 5 P.M. Australian Eastern Time
    USA: 12 A.M. Pacific Time

    Register for this webinar »

  • may 7th

    2. Identification of novel prion protein-based compounds to block the toxicity of Alzheimer’s Aβ oligomers

    Alzheimer’s disease is associated with progressive dementia and accumulation in the brain of the amyloid-β (Aβ) peptide, a cleavage product of the amyloid precursor protein. Compelling evidence suggests that soluble, oligomeric assemblies of Aβ are primarily responsible for the synaptic dysfunction underlying the cognitive decline in Alzheimer’s disease. Recently, the cellular prion protein has emerged as a novel and unexpected candidate receptor for Aβ oligomers.

    By applying several in vitro assays, we recently showed that a soluble, proteolytic fragment of the prion protein, called N1, binds to a precise assembly of Aβ oligomers with nanomolar affinity. We also report that N1 inhibits the polymerization of Aβ oligomers into amyloid fibrils, and suppress their neurotoxic effects in vitro and in vivo.

    Collectively, our data provide strong experimental evidence supporting the idea that N1, or small peptides derived from it, could be potent inhibitors of Aβ oligomer toxicity and represent an entirely new class of therapeutic agents for Alzheimer’s disease.

    Presenter
    Emiliano Biasini, PhD
    Instructor and Research Scientist
    Boston University
    U.S.

    May 7, 2013
    USA: 11 A.M. Eastern Time (8 A.M. Pacific Time)
    Europe: 5 P.M. Central Europe Time

    Register for this webinar »

  • june 4th

    3. Novel applications of histidine-tag capture using the ProteOn XPR36 system

    The ProteOn HTG sensor chip is functionalized with tris-NTA and is specifically designed for capturing histidine-tagged proteins. Jay Duffner, a senior scientist at Momenta Pharmaceuticals, will present some techniques for improving results in the measurement of binding kinetics of antibodies to histidine-tagged protein targets using the ProteOn system. Techniques discussed include capture conditions for histidine tagged proteins, reduction of non-specific binding, regeneration techniques and measurement of ligand activity over the course of an assay.

    Presenter
    Jay Duffner, PhD
    Senior Scientist
    Momenta Pharmaceuticals
    U.S.

    June 4, 2013
    USA: 11 A.M. Eastern Time (8 A.M. Pacific Time)
    Europe: 5 P.M. Central Europe Time

    Register for this webinar »

Quotes from previous webinar participants:

"The webinar offered me the opportunity to have a discussion with a wider audience on my current research."

Adriano Marchese, PhD
Associate Professor
Department of Pharmacology
Stritch School of Medicine
Loyola University Chicago
Chicago, Illinois

"It was valuable to us to summarize the data we have and look back on the problems we encountered in the initial experiments. Summarizing data for presentation is also a good way to inspire new ideas and assays that may be performed in the future."

Johan Nilvebrant, PhD student
School of Biotechnology
Department of Proteomics
Royal Institute of Technology (KTH)
AlbaNova University Center
Stockholm, Sweden

"The webinars introduced the latest applications of SPR technology for drug discovery workflows and provided valuable updates on the instrument's soft- and hardware."

Ralf J. Hosse, PhD
Group Leader Antibody Engineering
Roche Glycart
Schlieren, Switzerland

Webinar Speakers:

Olan Dolezal, PhD
Senior Research Scientist
CSIRO
Australia

Emiliano Biasini, PhD
Instructor and Research Scientist
Boston University
U.S.

Jay Duffner, PhD
Senior Scientist
Momenta Pharmaceuticals
U.S.

To view previous ProteOn XPR36 webinar series, please click here to visit our webinar library.

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