calendar icon: April 16

Using Mixed-Mode Selectivity to Address Purification Challenges

Learning objectives:

• Understanding mixed-mode chromatography basics and how to modulate

ligand/protein interactions

• Developing methods with the least amount of experimentation

• Address difficult purification challenges using new selectivity

DATE: April 16, 2014
TIME: 11:00 AM PST / 2:00 PM EST / 8:00 PM CET
SPEAKER: Xuemei He, PhD

Senior Staff Scientist, Process Chromatography Applications

Bio-Rad Laboratories

Abstract:

As the structures of novel biotherapeutics become more diverse, new purification schemes are emerging to effectively address purification challenges. Mixed-mode chromatography media with their unique selectivity are emerging as powerful tools for the removal of product related impurities. In this talk we discuss ligand-protein interactions and present several applications of mixed-mode chromatography, with a special focus on orthogonal approaches used to effectively remove closely related species while reducing feed manipulation and buffer consumption. Lastly, we will present proven approaches to accelerate method development by fine-tuning selectivity with minimal experimentation. The talk will highlight several cases spanning a range of proteins.

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calendar icon: April 24

Enabling Process Intensification Using Modern
Chromatography Media

Learning objectives:

• Developing purification processes with fewer chromatography unit operations

• Reducing feed manipulation and buffer consumption for a more compact process

• Exploiting novel selectivity to address purification challenges

DATE: April 24, 2014
TIME: 11:00 AM PST / 2:00 PM EST / 8:00 PM CET
SPEAKER: Priv-Doz Dr. Carsten Voss

Application Specialist, Process Chromatography

Bio-Rad Laboratories

Abstract:

The large scale production of biopharmaceuticals is continuing to evolve to meet the supply needs of the industry and ever increasing demands for value from healthcare systems. Bioproduction professionals are actively exploring and implementing several techniques such as disposable manufacturing, process analytical technology and quality by design to address these challenges. Concurrently, purification scientists are implementing new mixed-mode selectivity and high-capacity chromatography techniques to maximize overall process efficiency. In this talk we will highlight several examples in which creative use of chromatography has produced tangible benefits such as fewer unit operations, reduced buffer consumption, and processes with much higher recovery.

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Xuemei He, PhD
Senior Staff Scientist
Process Chromatography Applications
Bio-Rad Laboratories
USA
 

Headshot of Xuemei He

 
Priv-Doz Dr. Carsten Voss
Application Specialist
Process Chromatography
Bio-Rad Laboratories
USA
 

Headshot of Carsten Voss

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